Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors.
Identifieur interne : 005349 ( Main/Exploration ); précédent : 005348; suivant : 005350Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors.
Auteurs : Jieqing Zhu [République populaire de Chine] ; Gengfu Xiao ; Yanhui Xu ; Fang Yuan ; Congyi Zheng ; Yueyong Liu ; Huimin Yan ; David K. Cole ; John I. Bell ; Zihe Rao ; Po Tien ; George F. GaoSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Conformation des protéines, Dichroïsme circulaire, Escherichia coli (métabolisme), Glutathione transferase (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Peptides (), Protéines de fusion recombinantes (), Protéines de fusion recombinantes (métabolisme), Protéines de fusion virale (), Protéines de l'enveloppe virale (), Protéines du core viral (), Protéines virales (), Relation dose-effet des médicaments, Structure secondaire des protéines, Structure tertiaire des protéines, Température, Virus du SRAS (génétique).
- MESH :
- génétique : Virus du SRAS.
- métabolisme : Escherichia coli, Glutathione transferase, Protéines de fusion recombinantes.
- Conformation des protéines, Dichroïsme circulaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Peptides, Protéines de fusion recombinantes, Protéines de fusion virale, Protéines de l'enveloppe virale, Protéines du core viral, Protéines virales, Relation dose-effet des médicaments, Structure secondaire des protéines, Structure tertiaire des protéines, Température.
English descriptors
- KwdEn :
- Circular Dichroism, Dose-Response Relationship, Drug, Escherichia coli (metabolism), Glutathione Transferase (metabolism), Membrane Glycoproteins (chemistry), Peptides (chemistry), Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (metabolism), SARS Virus (genetics), Spike Glycoprotein, Coronavirus, Temperature, Viral Core Proteins (chemistry), Viral Envelope Proteins (chemistry), Viral Fusion Proteins (chemistry), Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Peptides, Recombinant Fusion Proteins, Viral Core Proteins, Viral Envelope Proteins, Viral Fusion Proteins, Viral Proteins.
- chemical , metabolism : Glutathione Transferase, Recombinant Fusion Proteins.
- genetics : SARS Virus.
- metabolism : Escherichia coli.
- Circular Dichroism, Dose-Response Relationship, Drug, Protein Conformation, Protein Structure, Secondary, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Temperature.
Abstract
Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.
DOI: 10.1016/j.bbrc.2004.04.141
PubMed: 15158473
Affiliations:
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Cole</name></author><author><name sortKey="Bell, John I" sort="Bell, John I" uniqKey="Bell J" first="John I" last="Bell">John I. Bell</name></author><author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name></author><author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Circular Dichroism</term><term>Dose-Response Relationship, Drug</term><term>Escherichia coli (metabolism)</term><term>Glutathione Transferase (metabolism)</term><term>Membrane Glycoproteins (chemistry)</term><term>Peptides (chemistry)</term><term>Protein Conformation</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>Recombinant Fusion Proteins (chemistry)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Temperature</term><term>Viral Core Proteins (chemistry)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Fusion Proteins (chemistry)</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Conformation des protéines</term><term>Dichroïsme circulaire</term><term>Escherichia coli (métabolisme)</term><term>Glutathione transferase (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Peptides ()</term><term>Protéines de fusion recombinantes ()</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines de fusion virale ()</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines du core viral ()</term><term>Protéines virales ()</term><term>Relation dose-effet des médicaments</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Température</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptides</term><term>Recombinant Fusion Proteins</term><term>Viral Core Proteins</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Transferase</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Glutathione transferase</term><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Circular Dichroism</term><term>Dose-Response Relationship, Drug</term><term>Protein Conformation</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>Spike Glycoprotein, Coronavirus</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term><term>Dichroïsme circulaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Peptides</term><term>Protéines de fusion recombinantes</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Protéines du core viral</term><term>Protéines virales</term><term>Relation dose-effet des médicaments</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GST-fusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Bell, John I" sort="Bell, John I" uniqKey="Bell J" first="John I" last="Bell">John I. Bell</name><name sortKey="Cole, David K" sort="Cole, David K" uniqKey="Cole D" first="David K" last="Cole">David K. Cole</name><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name><name sortKey="Liu, Yueyong" sort="Liu, Yueyong" uniqKey="Liu Y" first="Yueyong" last="Liu">Yueyong Liu</name><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><name sortKey="Yuan, Fang" sort="Yuan, Fang" uniqKey="Yuan F" first="Fang" last="Yuan">Fang Yuan</name><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhu, Jieqing" sort="Zhu, Jieqing" uniqKey="Zhu J" first="Jieqing" last="Zhu">Jieqing Zhu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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